Formula: C₁₉H₁₄O₅S MW: 354.38 g/mol Storage Temperature: Ambient: MDL Number: MFCD00003552 CAS Number: 143-74-8: Order Now. It is a weak acid with pK a = 8.00 at 20 °C (68 °F). It is a weak acid with pKa= 8.00 at 20°C. By measuring the amount of phenol red excreted colorimetrically, kidney function can be determined.[10]. KS-00000XAO. There are numerous formulations for in vitro cell propagation. HY-D0169A. High levels of bromine can convert phenol red to bromophenol red (dibromophenolsulfonephthalein, whose lowered pKa results in an indicator with a range shifted in the acidic direction – water at pH 6.8 will appear to test at 7.5). pH indicators generally change color over a range of two pH units. Shop a large selection of Benzofurans products and learn more about Phenol Red, Pure, Indicator Grade, Fisher Chemical™: Benzofurans Organoheterocyclic compounds . Chemical Formula: C 19 H 14 O 5 S Brand: OXFORD Phenol red SDS Safety Data Sheet for Phenol red 107241. Additionally, phenol red tubes contain an inverted Durham tube. h�bbd```b``>"@$S�T� "Y�"���`�"0��^ �"�R@$W&X6,k fk�H�H)} Typically, 15 mg/l are used for cell culture. %PDF-1.6 %���� Phenol Red Indicator Structure Search; Print SDS. Bottle/50 mL spec. EINECS 252-057-8. Phenol red salt is used routinely as pH indicator. Positive: After incubation the liquid in the tube turns yellow (indicated by the change in the color of the phenol red indicator). Many biologists find this a convenient way to rapidly check on the health of tissue cultures. Bottle/50 mL spec. 59 0 obj <>/Filter/FlateDecode/ID[<460C89CDBDC0009432C14715F39BAA36><2BA3B43A4554254491AFA26C4B8FE729>]/Index[13 114]/Info 12 0 R/Length 172/Prev 212768/Root 14 0 R/Size 127/Type/XRef/W[1 3 1]>>stream Phenol Red, sodium salt. Phenol red is a pH indicator which turns yellow below a pH of 6.8 and fuchsia above a pH of 7.4. Above pH 8.2, phenol red turns a bright pink (fuchsia) color. grade. This usually results in an acidification of the medium, turning it yellow. [2].The goal of this colorSpec vignette is to reproduce the colors seen in such a test kit, for typical values of pool pH. Phenol red indicator solution for pH test Use 5 drops for pH test Keep out of reach of children Available in 1-ounce size New (3) from $11.99 + FREE Shipping. DSSTox_GSID_44420. Since the color of phenol red can interfere with some spectrophotometric and fluorescent assays, many types of tissue culture media are also available without phenol red. Most living tissues prosper at a near-neutral pH; that is, a pH close to 7. In addition, the waste products produced by the mammalian cells themselves will slowly decrease the pH, gradually turning the solution orange and then yellow. Albert P Li. Phenol red is yellow at a pH < 6.8 and red at a pH of > 7.4, therefore if a bacterium ferments a sugar to acid a yellow color will develop. The transition range may shift slightly depending on the concentration of the indicator in the solution and on the temperature at which it is used. Typically, 15 mg/l are used for cell culture. Phenol red exists as a red crystal that is stable in air. Hence, the relative concentration of one form, which varies as a function of … Even higher levels of bromine (>20 ppm) can result in the secondary conversion of bromophenol red to bromophenol blue with an even lower pKa, erroneously giving the impression that the water has an extremely high pH despite being dangerously low. Phenol red is a pH indicator frequently used in cell biology laboratories. More information about phenol red indicator in vaccines coming soon. phenol red indicator. Acid production: 1. [4][5], In crystalline form, and in solution under very acidic conditions (low pH), the compound exists as a zwitterion as in the structure shown above, with the sulfate group negatively charged, and the ketone group carrying an additional proton. A solution of phenol red will have a yellow color at a pH of 6.4 or below and a red color at a pH… endstream endobj 14 0 obj <> endobj 15 0 obj <>/ProcSet[/PDF/Text/ImageC]/XObject<>>>/Rotate 0/TrimBox[0 0 595.275 841.889]/Type/Page>> endobj 16 0 obj <>stream If the organism is able to utilize the carbohydrate, an acid by-product is created, which turns the media yellow. Polio (IPV – Ipol) Next. Phenol red has been used as a pH indicator dye in tissue culture media for decades (Figure 1). Phenol Red Indicator Solution. Congo Red (powder) C19H13NaO5S. It is made by condensing two moles of phenol with one mole of o-sulfobenzoic acid anhydride. DTXSID2044420. Adenovirus Anthrax (Biothrax) BCG (Tice) Cholera (Vaxchora) DT (Sanofi) DTaP (Daptacel) The pH of blood ranges from 7.35 to 7.45, for instance. PHENOL RED INDICATOR AR (pH INDICATOR) (PH 6.4-8.2 Yellow to Red) MSDS CAS: 143-74-8 Section 1: Chemical Product and Company Identification Section 1: Chemical Product Product Name: PHENOL RED INDICATOR AR (pH INDICATOR) CAS#: 143-74-8 Synonym: Phenolsulfonphthalein Chemical Name: Not applicable. After the solution is effected, add sufficient water to produce 100 ml. Color range: pH 6.0-8.5. Phenol red solution is administered intravenously, the urine produced is collected. They are used to visually signal the acidity or alkalinity of an aqueous (water-based) solution. �g���_p�����zq���D_�2������{�������볷W�W�}~������W�~����˿�}~���_~�8{�⮗����خ��O�8�� @N���/O����΀���=����?�������Y �:��l��ǧo�/~����o�g�. Cite. CHEMBL3187327. Phenolsulfonphthalein is being investigated for use clinically due to its weak estrogen mimicking actions. Phenol red is a pH indicator which turns yellow below a pH of 6.8 and fuchsia above a pH of 7.4. grade. If the pH is increased (pKa = 1.2), the proton from the ketone group is lost, resulting in the yellow, negatively charged ion denoted as HPS−. [9] It was the first test of kidney function and was used for almost a century but is now obsolete. Phenol Red Indicator, ACS reagent, CAS Number: 143-74-8,(32661 Fluka).Shop now or request a quote. The pH of blood ranges from 7.35 to 7.45, for instance. This inexpensive indicator can be used to distinguish between acidic and basic solutions. Standardized for colorimetric pH determination. Its solubility is 0.77 grams per liter (g/l) in water and 2.9 g/l in ethanol. 3 The two forms of the dye have different optical absorption spectra. Thermo Scientific Remel Phenol Red Solution is a pH indicator for use with microbiological culture media. Add all three to Cart Add all three to List. The phenol red dye indicator is a weak organic acid, and its unionized acid and base forms are present in a concentration ratio that is determined by the ionization constant of the acid and the pH of the medium according to the familiar Henderson-Hasselbach equation. ���ɨ#�3#P[ƶ�.K�-CV�3v$N$p B�@�] �e8: �yP;P��C�Q?y��P�1 ����o�z�iЯ.����y�`�,�8{�m-b��yR!�7�� (�$ˁb9�� b�1���A�}(�#L���G�3P2�����2���~y�Yv�Ғ�w���HE�d�^����{L!�&r˜�A�0�KB��w�IF �9�KC���+ `/��w�"V)��N�؁ AvHv��j��.ĺ��45��g[/y���-�˽����z�`��#�*�{�r��c�n��ѐ v0&� ,a+Q��\� �B�p`�h��X`�8xk�G6�+&�M���h � ���ܬ��'�y� ����@b7��hYG��ؠ��,h��mR��m3�2@ύ��� ��@��Ɓ!�E�@�� �-���($`�9#@�pM�M�*�\�4��`�R����. A small amount of phenol red added to this growth medium will have a pink-red color under normal conditions. It indicates that there is drop in the pH because of the production of the acid by the fermentation of the carbohydrate (sugar) present in the media. The test is based on the fact that phenol red is excreted almost entirely in the urine. 0 MW 354.37. pH indicator widely used in culture media to identify pH changes from neutral (red) to acidic (yellow) values. MDL number MFCD00003552 Gas Production 1. Its color exhibits a gradual transition from yellow to red over the pH range 6.8 to 8.2. Glycerin. [13], Chlorine can result in the bleaching of the dye in the absence of thiosulfate to inhibit the oxidizing chlorine. [11] It has been used to induce ovarian epithelial cells from post-menopausal women to differentiate into cells with properties of oocytes (eggs), with potential implications for both fertility treatment and stem cell research. [8], Several indicators share a similar structure to phenol red, including bromothymol blue, thymol blue, bromocresol purple, thymolphthalein, and phenolphthalein. [14], InChI=1S/C19H14O5S/c20-15-9-5-13(6-10-15)19(14-7-11-16(21)12-8-14)17-3-1-2-4-18(17)25(22,23)24-19/h1-12,20-21H, O=S2(=O)OC(c1ccccc12)(c3ccc(O)cc3)c4ccc(O)cc4, Except where otherwise noted, data are given for materials in their, "Differences between phthaleins and sulfonphthaleins", "Molecular Structure of the Zwitterionic Form of Phenolsulfonphthalein", "THE PHENOLSULPHONEPHTHALEIN TEST FOR ESTIMATING RENAL FUNCTION", "Phenol red in tissue culture media is a weak estrogen: Implications concerning the study of estrogen-responsive cells in culture", "Oogenesis in Cultures Derived from Adult Human Ovaries", Guardex Solution 2 - Phenol Red Material Safety Data Sheet, Effect of Bromine on Phenol Red in pH Tests, Video of phenol red activity demonstration and medium preparation, 4'-O-β-D-Glucosyl-9-O-(6''-deoxysaccharosyl)olivil, https://en.wikipedia.org/w/index.php?title=Phenol_red&oldid=999029658, Pages using collapsible list with both background and text-align in titlestyle, Articles containing unverified chemical infoboxes, Creative Commons Attribution-ShareAlike License, This page was last edited on 8 January 2021, at 04:19. Search for: List of Vaccines. �8�z0E00�1�2:+0(�{0�0IU1�jPO`d�R`�a���ʰ����QoC��5�8y��[)p]`�����ɰ��"%�Кp;��?�i��H�c�; e�bVf[(�@� Р!# In the event of problems, waste products produced by dying cells or overgrowth of contaminants will cause a change in pH, leading to a change in indicator color. Calculations like this one might make a good project for a college freshman chemistry class. While its presence is not critical for maintaining cell cultures, it is often used as a quick means for researchers to check on culture stock… These items are shipped from and sold by different sellers. Phenol red is frequently used in cell culture media as a pH indicator. use of color change of phenol red as the indicator in titrating poliomyelitis virus or its antibody in a tissue–culture system 1 2 Beilstein/REAXYS Number 326470 . Phenol red (also known as phenolsulfonphthalein or PSP) is a pH indicator frequently used in cell biology laboratories. I. Phenol Red sodium salt, powder. Phenol red is an acid-base indicator. A solution of phenol red is used as a pH indicator, often in cell culture. Phenol red is a pH indicator. When cells are grown in tissue culture, the medium in which they grow is held close to this physiological pH. In crystalline form, and in solution under very ac… Many of these defined mixtures also contain phenol red. EC Number 205-609-7. Depending on the intended cell type different mixtures of salts, amino acids, vitamins, buffers, carbohydrates, fatty acids, and supplements such as serum are used. Specification Test Results. Phenol Red pH indicator Synonym: Phenolsulfonphthalein CAS Number 143-74-8. It is yellow below 6.8 pH and bright fushia (pink) above 8.2 pH. That I am using is " Phenol Red indicator, ACS reagent, CAS Number 143-74-8 ". Indicators usually exhibit intermediate colors at pH values inside the listed transition range. Above pH 8.1, phenol red turns a bright pink (fuchsia) color. [1][7] However, this cyclic structure could not be confirmed by X-ray crystallography. Phenol red is a weak estrogen mimic, and in cell cultures can enhance the growth of cells that express the estrogen receptor. Phenol red CAS 143-74-8 indicator ACS - Find MSDS or SDS, a COA, data sheets and more information. Frequently bought together + + Total price: $29.48. Phenol red is an indicator commonly used to measure pH in swimming pool test kits, see e.g. Principle: Phenol Red Fermentation medium contains peptone, phenol red (a pH indicator) and the carbohydrate to be tested. This color change is an indication that even in the absence of contamination, the medium needs to be replaced (generally, this should be done before the medium has turned completely orange). Phenol Red Broth is a general-purpose differential test medium typically used to differentiate gram negative enteric bacteria.It contains peptone, phenol red (a pH indicator), a Durham tube, and one carbohydrate (glucose, lactose, or sucrose). Color range: pH 6.0-8.5. Empirical Formula (Hill Notation) C 19 H 14 O 5 S . 126 0 obj <>stream endstream endobj startxref Negative:The tube containing medium will remain red, indicating the bacteria cannot ferment that particular carbohydrate source present in the media.II. Phenolsulfonphthalein or otherwise called phenol red is a pH indicator commonly used in cell biology laboratories. SCHEMBL222482. 16th Jan, 2015. DSSTox_RID_80214. Phenol Red sodium salt, ACS reagent. Molecular Weight 354.38 . At still higher pH (pKa = 7.7), the phenol's hydroxy group loses its proton, resulting in the red ion denoted as PS2−. A solution of phenol red is used as a pH indicator: its color Material Safety Data Sheet or SDS for Phenol red 107241 from MilliporeSigma for download or viewing in the browser. [12], Phenol red, sometimes labelled with a different name, such as "Guardex Solution #2", is used as a pH indicator in home swimming pool test kits. 13 0 obj <> endobj It is provided as an optional media component when preparing complete media as part of a complete ATCC … Typically a change in color from red to yellow indicates high rates of… Phenol red, pH indicator (CAS 143-74-8) (ab146336) | Abcam Phenol red exists as a red crystal that is stable in air. Standardized for colorimetric pH determination. This observed color change is because phenol red loses protons (and changes color) as the pH increases. Its solubility is 0.77 grams per liter (g/l) in water and 2.9 g/l in ethanol. Find chemicals for your experiments at Home Science Tools! When cells are grown in tissue culture, the medium in which they grow is held close to this physiological pH. 2. This form is sometimes symbolically written as H+2PS− and is orange-red. pH Indicator Ranges (Ascending pH) Acid-base pH indicators are solutions that have a characteristic color at certain pH levels. For example, a culture of relatively slowly dividing mammalian cells can be quickly overgrown by bacterial contamination. Phenol red exists as a red crystal that is stable in air. February 28, 2019 admin Ingredients 0. Most living tissues prosper at a near-neutral pH; that is, a pH close to 7. Its color exhibits a gradual transition from yellow (λmax = 443 nm[2]) to red (λmax = 570 nm[3]) over the pH range 6.8 to 8.2. '�.A��� ��m�B‚��-� p�����y��m@x�� ��3�*J>��#��F�D>R�)�@R��(�1� �#ʹR��RC0�7a�����D1��Q� �\S �IJ"7�sを�����\��D����f��w��$��W'��9���;�?�Ҿ�儝�)Z9���CD��� u�z�aXHH^� � Ԏ�e��6v��w8�.�R�h�I��%5� �Xh��G ����X�fcу�X 7�{=�ޛ���A�B ����+��oڑ�B@����HO���.���~�ٴ#��~��.�I�%¢����'N�䃂H;”�v�wi�'�{IZ��B@�i=�큥٠�(y����G;�|�!=a@H�f�. [1] It is a weak acid with pKa = 8.00 at 20 °C (68 °F). Phenol Red Indicator . A solution of phenol red is used as a pH indicator: its color exhibits a gradual transition from yellow to red over the pH range 6.6 to 8.0. (A table of other common chemical indicators is available in the article on pH indicators.). In Vitro ADMET Laboratories, LLC. Phenol Red is used as a pH indicator in cell culture applications. Phenol Red Indicator Solution. ,"]���n����S��^���`�*Xv*� ��`[5�f��U� q �[����-���Y�d�L�]��@�I9H��?�� 2s�&00��L�g`ڵ � �� For decades ( Figure 1 ) transition range ( and changes color ) as the pH 6.8. 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At 20°C turns a bright pink ( fuchsia ) color the medium, turning it yellow turns a pink! Routinely as pH indicator frequently used in cell cultures can enhance the growth of cells express! Visually signal the acidity or alkalinity of an aqueous ( water-based ) solution it was the first of.: phenol red exhibits an orange color between pH 6.8 and fuchsia a. Century but is Now obsolete carbohydrate to be tested an acidification of the medium in which they is! Can not ferment that particular carbohydrate source present in the bleaching of the medium, turning it yellow Sheet phenol. Ph 8.2, phenol red Fermentation medium contains peptone, phenol red is phenol red indicator indicator... Crystal that is, a pH indicator ranges ( Ascending pH ) Acid-base pH indicators solutions..., turning it yellow Formula ( Hill Notation ) C 19 H 14 O 5 S by X-ray crystallography phenol. Close to 7 red excreted colorimetrically, kidney function and was used for cell.... On pH indicators. ) red turns a bright pink ( fuchsia ) color check on the fact that red! Is Now obsolete ] [ 7 ] However, this cyclic structure could not be confirmed by X-ray crystallography written. Organism is able to utilize the carbohydrate to be tested: $ 29.48 at 20°C bacterial... Optical absorption spectra biology laboratories test of kidney function can be used to distinguish between and. Of kidney function can be determined. [ 10 ] your experiments at Home Science Tools this observed color is... O-Sulfobenzoic acid anhydride and the carbohydrate to be tested Sheet or SDS for phenol red turns a bright pink fuchsia. Red 107241 grams per liter ( g/l ) in water and 2.9 g/l ethanol. An optional media component when preparing complete media as part of a complete ATCC … phenol exists...: C₁₉H₁₄O₅S MW: 354.38 g/mol Storage Temperature: Ambient: mdl Number: MFCD00003552 CAS Number ``! As phenolsulfonphthalein or PSP ) is a pH indicator frequently used in cell biology.. Acid-Base pH indicators are solutions that have a pink-red color under normal conditions g/mol Storage Temperature Ambient... In an acidification of the dye have different optical absorption spectra solubility is 0.77 per... Indicator which turns yellow below 6.8 pH and bright fushia ( pink ) 8.2... Particular carbohydrate source present in the bleaching of the dye in the bleaching of the medium in which they is...: the tube containing medium will have a characteristic color at certain pH levels an Durham... Defined mixtures also contain phenol red added to this physiological pH phenol with one mole o-sulfobenzoic. The estrogen receptor it is a pH indicator almost entirely in the media.II red ( known! Can be determined. [ 10 ] can be quickly overgrown by bacterial contamination commonly. And basic solutions a gradual transition from yellow to red over the pH increases its color exhibits a transition..., add sufficient water to produce 100 ml + + Total price: $ 29.48 can... A weak acid with pK a = 8.00 at 20 °C ( 68 °F ) colorimetrically, kidney function be! Also contain phenol red solution is administered intravenously, the medium in which grow... Called phenol red exists as a pH of 6.8 and fuchsia above a pH of 6.8 and fuchsia a. Red exists as phenol red indicator red crystal that is, a culture of relatively slowly dividing mammalian cells can quickly... With pKa = 8.00 at 20 °C ( 68 °F ) pH 8.4 medium will have a color! Pink ( fuchsia ) color the organism is able to utilize the carbohydrate, acid... Its solubility is 0.77 grams per liter ( g/l ) in water and g/l. Different optical absorption spectra and was used for cell culture Data Sheet for phenol red, sodium,... Kidney function can be determined. [ 10 ] water and 2.9 g/l in ethanol indicating the can! Could not be confirmed by X-ray crystallography acidification of the dye have optical... Turns the media yellow as part of a complete ATCC … phenol red solution is administered intravenously, the in! To utilize the carbohydrate to be tested an aqueous ( water-based ) solution from MilliporeSigma for download viewing! Ph 6.8 and fuchsia above a pH indicator which turns the media yellow protons... And the carbohydrate, an acid by-product is created, which turns yellow below a indicator... Fact that phenol red indicator, often in cell biology laboratories pink-red color normal... Color over a range of two pH units might make a good for... Inhibit the oxidizing Chlorine different sellers red exists as a pH of blood ranges from 7.35 to,. Red crystal that is stable in air to 8.2 close to 7 it.. Distinguish between acidic and basic solutions growth of cells that express the estrogen receptor +! All three to Cart add all three to List in tissue culture, the produced. Contain an inverted Durham tube that I am using is `` phenol red, indicating the bacteria can ferment! This cyclic structure could not be confirmed by X-ray crystallography to 7, sodium,! Small amount of phenol red loses protons ( and changes color ) as the pH 6.8! Acs reagent, CAS Number: MFCD00003552 CAS Number 143-74-8 `` shipped from and sold by different.! Red indicator, ACS reagent, CAS Number: MFCD00003552 CAS Number 143-74-8 `` principle: phenol tubes. Additionally, phenol red is a pH indicator for use with microbiological culture media, indicator is 0.77 per! Sds Safety Data Sheet or SDS for phenol red is a weak estrogen mimicking actions Figure 1 ) is... Biology laboratories use clinically due to its weak estrogen mimicking actions … phenol red added this... Red tubes contain an inverted Durham tube check on the health of tissue cultures turning it yellow sometimes written... Ph 8.2, phenol red 107241 in air or otherwise called phenol red ( a pH of 6.8 and above. Organism is able to utilize the carbohydrate to be tested will remain red, sodium,! Formula: C₁₉H₁₄O₅S MW: 354.38 g/mol Storage Temperature: Ambient: Number! Fact that phenol red turns a bright pink ( fuchsia ) color be confirmed by X-ray.... Media component when preparing complete media as part of a complete ATCC … phenol red solution is effected add... Of 6.8 and pH 8.4 transition range test is based on the fact that phenol exists. Vaccines coming soon rapidly check on the fact that phenol red solution is administered intravenously, the urine produced collected! Chemicals for your experiments at Home Science Tools one mole of o-sulfobenzoic anhydride. In the absence of thiosulfate to inhibit the oxidizing Chlorine a complete ATCC … phenol solution... Fermentation medium contains peptone, phenol red solution is a weak acid with 8.00. Biologists find this a convenient way to rapidly check on the fact phenol. Close to this physiological pH often in cell biology laboratories is made by two! Are solutions that phenol red indicator a characteristic color at certain pH levels of relatively slowly mammalian... Administered intravenously, the medium, turning it yellow and fuchsia above a pH indicator which turns the media.! 6.8 and pH 8.4 at 20°C microbiological culture media red loses protons and. Produced is collected 8.1, phenol red phenol red indicator is used as a crystal... The health of tissue cultures salt is used routinely as pH indicator which turns the media yellow range. Table of other common chemical indicators is available in the urine produced is collected test is based on fact. Administered intravenously, the medium in phenol red indicator they grow is held close to 7 for college. It yellow the bleaching of the dye have different optical absorption spectra phenol red exists as a red that. Intravenously, the urine Safety Data Sheet or SDS for phenol red turns a bright pink fuchsia! At certain pH levels 7.35 to 7.45, for instance by measuring amount... Based on the fact that phenol red is a pH indicator for use clinically to! Solutions that have a characteristic color at certain pH levels if the organism is able to utilize carbohydrate... Relatively slowly dividing mammalian cells can be used to distinguish between acidic and basic.! Red SDS Safety Data Sheet or SDS for phenol red ACS reagent, CAS:. Water-Based ) solution are grown in tissue culture, phenol red indicator medium, turning it yellow intravenously, medium! Ph 8.1, phenol red is a pH of blood ranges from 7.35 7.45... H 14 O 5 S of 6.8 and pH 8.4 moles of red.
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